Rabies titer testing: RFFIT and FAVN explained

A rabies titer test measures antibodies after vaccination. Before taking a blood sample, confirm your destination’s accepted test method, laboratory, and timing. An unsuitable test can delay travel by months.

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Compare the tests

RFFIT and FAVN measure antibodies that neutralise the rabies virus, with results reported in IU/ml. Acceptance depends on the destination’s rules and the laboratory’s approval, as well as the test method.

ComparisonRFFIT testFAVN test
What is it?Rapid Fluorescent Focus Inhibition Test.Fluorescent Antibody Virus Neutralisation test.
Required resultSet by the destination; a common travel threshold is at least 0.5 IU/ml.A common travel threshold is at least 0.5 IU/ml. A result of 0.49 is below that threshold.
Acceptance for travelSuitable where the receiving authority accepts the method and laboratory. An unapproved local test is not an automatic substitute.Used by pet travel programmes that accept it, subject to laboratory approval, timing, and documentation.
LaboratoryConfirm in advance that both the laboratory and method are accepted by the destination.Choose a laboratory approved or designated by the receiving authority, using its current list.
If FAVN is specifically requiredDo not substitute another method without the competent authority’s agreement.Obtain the official report and ensure the sample records match your pet’s microchip number.

Start with the destination’s requirements, then choose the test and laboratory. Not every titer test is accepted for travel, and FAVN is not the only method accepted by every country.

Steps for EU travel

This outlines the usual non-commercial travel process where rabies testing is required. Commercial shipments and exempt cases may follow different procedures.

  1. First

    Microchip identification

    Have the chip implanted or read and recorded before rabies vaccination. Use the same number throughout the paperwork.

  2. From 12 weeks of age

    Rabies vaccination

    An authorised vet administers the vaccine. A primary vaccination becomes valid at least 21 days after completion.

  3. At least 30 days after primary vaccination

    Blood sampling

    Your vet collects and identifies the sample. Follow the laboratory’s instructions for serum volume, storage, and shipment.

  4. Turnaround varies by laboratory

    Testing and official report

    Use a designated laboratory and obtain a result of at least 0.5 IU/ml. Include the report with the health certificate.

  5. At least 90 days from sampling

    Waiting period

    Where required, this interval must pass before the health certificate is issued. Count from sampling, not the result date.

  6. Within 10 days before entry checks

    Health certificate

    Arrange completion and endorsement by the competent authority, then check the paperwork and its validity before travelling.

Before planning travel from Egypt, check the destination’s current departure-country classification and exemptions. Requirements can depend on the route and type of transport.

Destination waiting periods

These planning intervals apply to routes requiring testing and are not the full preparation time. Check departure-country eligibility, vaccinations, permits, and quarantine requirements separately.

DestinationWaiting periodStarting point and conditions
European Union — where testing is requiredAt least 90 daysFrom sampling to health certificate issue, with the required vaccination timing and a designated laboratory.
Great Britain — unlisted countries3 monthsFrom sampling; collect at least 30 days after vaccination and use an EU-approved laboratory.
Japan — non-designated regionsAt least 180 daysFrom a successful sample after the second vaccination, with sampling counted as day zero. Other import requirements also apply.
Australia — routes requiring RNATTAt least 180 daysFrom laboratory receipt of the sample yielding an acceptable result. Check export-country eligibility, identity verification, and permits.
Hawaii — direct airport release or 5 Day Or Less programmeAt least 30 daysFrom laboratory receipt of a successful FAVN sample, subject to all programme, vaccination, and document requirements.
New ZealandDepends on country category and applicable standardConfirm the test window and waiting period in the current MPI standard before finalising travel dates.

The clock starts at different points depending on the destination: blood collection or laboratory receipt. Do not use the result date in place of either.

Results and common mistakes

If the result is below 0.5

  • It does not meet a travel requirement of at least 0.5 IU/ml. The result alone does not determine your pet’s overall health.
  • Your vet reviews the vaccination record and your pet’s health, then decides whether a booster and repeat test are needed.
  • The waiting period may need to restart from the new successful sample, depending on destination rules.

Mistakes that can delay travel

  • Microchipping after vaccination, or inconsistent chip numbers on documents.
  • Taking the blood sample before the destination allows it.
  • Choosing an unaccepted test method or laboratory.
  • Incorrect sample handling or failure to follow laboratory instructions, potentially causing rejection.
  • Allowing the previous rabies vaccination to expire before a booster.

Official sources

This guide supports planning and does not replace advice from your vet or the destination’s official authority. Check requirements before sampling and booking; rules and exemptions can change.

Need help choosing the test and timing?

Tell us your destination, pet species, and travel date. Our team can help you organise the next steps.

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